Journal
JOURNAL OF VASCULAR RESEARCH
Volume 46, Issue 4, Pages 347-352Publisher
KARGER
DOI: 10.1159/000189795
Keywords
Atherosclerosis; Atherosclerotic lesions; apoE(-/-) mice; Plaque quantification; Lysochrome; Oil Red O
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Funding
- Korea Science and Engineering Foundation [F01-2006-000-10172-0]
- Korea Research Foundation [KRF-2007-313-C00810]
- Korean Food Research Institute
- National Research Foundation of Korea [2007-313-C00810] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The quantification of aortic lesions is an important endpoint analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification. Copyright (C) 2009 S. Karger AG, Basel
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