Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 45, Pages E6129-E6138Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1505799112
Keywords
virus-mimetic vesicle; vaccine; nanobiotechnology; antigen delivery system; cell membrane
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Funding
- Ministry of Science and Technology of China (MOST) [2014CB744503, 2013CB733802, 2012DFH30020]
- National Natural Science Foundation of China (NSFC) [81422023, 51273165, 81101101, 81371596, 81371817]
- Program for New Century Excellent Talents in University [NCET-13-0502]
- Fundamental Research Funds for the Central Universities [2013121039, 20720150141]
- Intramural Research Program, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
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It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses.
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