Journal
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B
Volume 27, Issue 6, Pages 3088-3091Publisher
A V S AMER INST PHYSICS
DOI: 10.1116/1.3259953
Keywords
adhesion; chromium; elastomers; gold; nanofabrication; proteins
Funding
- National Institutes of Health through the NIH Roadmap for Medical Research [PN2 EY016586]
- NSF [CHE-0641523, ECS0335765]
- New York State Office of Science, Technology, and Academic Research (NYSTAR)
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The authors describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited, while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.
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