Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 26, Pages 8130-8135Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1504951112
Keywords
basic helix loop helix; Catharanthus roseus; jasmonate; Madagascar periwinkle; iridoids
Categories
Funding
- European Union Seventh Framework Program FP7 [222716-SMARTCELL]
- Short-Term Scientific Missions (STSM) program from European Union COST Action [FA1006-PlantEngine]
- Research Foundation Flanders [G005212N]
- European Molecular Biology Organization (EMBOCOFUND)
- Marie Curie Actions [GA-2010-267154]
- European Union COST Action [FA1006-PlantEngine]
- Swiss National Foundation
- John Innes Centre
- Advanced Human Capital Program (Becas Chile) [73140628-CONICYT]
- Directorate General of Higher Education of the Republic Indonesia
- French Space Agency [Centre National d'Etudes Spatiales/Centre Spatial Guyanais (CNES/CSG)]
- RNA-Seq
- BBSRC [BB/J009091/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [1100175, BB/J009091/1] Funding Source: researchfish
Ask authors/readers for more resources
Plants make specialized bioactive metabolites to defend themselves against attackers. The conserved control mechanisms are based on transcriptional activation of the respective plant species-specific biosynthetic pathways by the phytohormone jasmonate. Knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. By transcriptome analysis and functional screens in the medicinal plant Catharanthus roseus (Madagascar periwinkle), the unique source of the monoterpenoid indole alkaloid (MIA)-type anticancer drugs vincristine and vinblastine, we identified a jasmonate-regulated basic helix-loop-helix (bHLH) transcription factor from clade IVa inducing the monoterpenoid branch of the MIA pathway. The bHLH iridoid synthesis 1 (BIS1) transcription factor transactivated the expression of all of the genes encoding the enzymes that catalyze the sequential conversion of the ubiquitous terpenoid precursor geranyl diphosphate to the iridoid loganic acid. BIS1 acted in a complementary manner to the previously characterized ethylene response factor Octadecanoid derivative-Responsive Catharanthus APETALA2-domain 3 (ORCA3) that transactivates the expression of several genes encoding the enzymes catalyzing the conversion of loganic acid to the downstream MIAs. In contrast to ORCA3, overexpression of BIS1 was sufficient to boost production of high-value iridoids and MIAs in C. roseus suspension cell cultures. Hence, BIS1 might be a metabolic engineering tool to produce sustainably high-value MIAs in C. roseus plants or cultures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available