Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 28, Pages 8656-8660Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1506351112
Keywords
centromere assembly; centromeric cohesion; ploidy stability; Arabidopsis; MZT1
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Funding
- Centre National de la Recherche Scientifique (CNRS)
- Ministsre de l'Enseignement Superieur et de la Recherche (MESR)
- Partenariats Hubert Curien programs [31504VJ, ALW2PJ/09053]
- Netherlands' Organization for Scientific Research
- Deutscher Akademischer Austausch Dienst
- MESR
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Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly conserved gamma-tubulin complex protein 3-interacting proteins (GIPs) in Arabidopsis centromere regulation. We show that GIPs form a complex with CENH3 in cycling cells. GIP depletion in the gip1gip2 knockdown mutant leads to a decreased CENH3 level at centromeres, despite a higher level of Mis18BP1/KNL2 present at both centromeric and ectopic sites. We thus postulate that GIPs are required to ensure CENH3 deposition and/or maintenance at centromeres. In addition, the recruitment at the centromere of other proteins such as the CENP-C kinetochore component and the cohesin subunit SMC3 is impaired in gip1gip2. These defects in centromere architecture result in aneuploidy due to severely altered centromeric cohesion. Altogether, we ascribe a central function to GIPs for the proper recruitment and/or stabilization of centromeric proteins essential in the specification of the centromere identity, as well as for centromeric cohesion in somatic cells.
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