Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 38, Pages E5308-E5317Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1514475112
Keywords
neurodegeneration; Parkinson's disease; synucleinopathies; strains
Categories
Funding
- National Institutes of Health [AG021601, AG002132, AG010770, AG031220]
- Sherman Fairchild Foundation
- Rainwater Charitable Foundation
- Mary Jane Brinton Fund
- Parkinson's UK, a charity registered in England and Wales [948776]
- Parkinson's UK, a charity registered in Scotland [SC037554]
- Sydney Brain Bank
- Neuroscience Research Australia
- University of New South Wales
- National Health and Medical Research Council of Australia
- Parkinson's UK [G-0909, J-1402] Funding Source: researchfish
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Prions are proteins that adopt alternative conformations that become self-propagating; the PrPSc prion causes the rare human disorder Creutzfeldt-Jakob disease (CJD). We report here that multiple system atrophy (MSA) is caused by a different human prion composed of the alpha-synuclein protein. MSA is a slowly evolving disorder characterized by progressive loss of autonomic nervous system function and often signs of parkinsonism; the neuropathological hallmark of MSA is glial cytoplasmic inclusions consisting of filaments of alpha-synuclein. To determine whether human alpha-synuclein forms prions, we examined 14 human brain homogenates for transmission to cultured human embryonic kidney (HEK) cells expressing full-length, mutant human alpha-synuclein fused to yellow fluorescent protein (alpha-syn140*A53T-YFP) and TgM83(+/-) mice expressing alpha-synuclein (A53T). The TgM83(+/-) mice that were hemizygous for the mutant transgene did not develop spontaneous illness; in contrast, the TgM83(+/+) mice that were homozygous developed neurological dysfunction. Brain extracts from 14 MSA cases all transmitted neurodegeneration to TgM83(+/-) mice after incubation periods of similar to 120 d, which was accompanied by deposition of alpha-synuclein within neuronal cell bodies and axons. All of the MSA extracts also induced aggregation of alpha-syn*A53T-YFP in cultured cells, whereas none of six Parkinson's disease (PD) extracts or a control sample did so. Our findings argue that MSA is caused by a unique strain of alpha-synuclein prions, which is different from the putative prions causing PD and from those causing spontaneous neurodegeneration in TgM83(+/+) mice. Remarkably, alpha-synuclein is the first new human prion to be identified, to our knowledge, since the discovery a half century ago that CJD was transmissible.
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