Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 27, Pages E3609-E3618Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1507704112
Keywords
adenosine A(2A) receptor; dopamine D-2 receptor; caffeine; GPCR heteromers
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Funding
- Government of Catalonia [2009-SGR-12]
- Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas [CB06/05/0064]
- Spanish Ministerio de Ciencia y Teconolgia [SAF 2011-23813]
- National Institute on Drug Abuse
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Adenosine A(2A) receptor (A(2A)R)-dopamine D-2 receptor (D2R) heteromers are key modulators of striatal neuronal function. It has been suggested that the psychostimulant effects of caffeine depend on its ability to block an allosteric modulation within the A(2A)R-D2R heteromer, by which adenosine decreases the affinity and intrinsic efficacy of dopamine at the D2R. We describe novel unsuspected allosteric mechanisms within the heteromer by which not only A(2A)R agonists, but also A(2A)R antagonists, decrease the affinity and intrinsic efficacy of D2R agonists and the affinity of D2R antagonists. Strikingly, these allosteric modulations disappear on agonist and antagonist coadministration. This can be explained by a model that considers A(2A)R-D2R heteromers as heterotetramers, constituted by A(2A)R and D2R homodimers, as demonstrated by experiments with bioluminescence resonance energy transfer and bimolecular fluorescence and bioluminescence complementation. As predicted by the model, high concentrations of A(2A)R antagonists behaved as A(2A)R agonists and decreased D2R function in the brain.
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