Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 8, Pages E891-E900Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1415488112
Keywords
coral snake toxin; MmTX1; MmTX2; GABA(A) receptor; hippocampal neurons
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Funding
- Centre National de la Recherche Scientifique
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GABA(A) receptors shape synaptic transmission by modulating Cl-conductance across the cell membrane. Remarkably, animal toxins that specifically target GABA(A) receptors have not been identified. Here, we report the discovery of micrurotoxin1 (MmTX1) and MmTX2, two toxins present in Costa Rican coral snake venom that tightly bind to GABA(A) receptors at subnanomolar concentrations. Studies with recombinant and synthetic toxin variants on hippocampal neurons and cells expressing common receptor compositions suggest that MmTX1 and MmTX2 allosterically increase GABA(A) receptor susceptibility to agonist, thereby potentiating receptor opening as well as desensitization, possibly by interacting with the alpha(+)/beta(-) interface. Moreover, hippocampal neuron excitability measurements reveal toxin-induced transitory network inhibition, followed by an increase in spontaneous activity. In concert, toxin injections into mouse brain result in reduced basal activity between intense seizures. Altogether, we characterized two animal toxins that enhance GABAA receptor sensitivity to agonist, thereby establishing a previously unidentified class of tools to study this receptor family.
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