Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 46, Pages 14266-14271Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1518628112
Keywords
electron; cryotomography; platelet; microtubule; cancer
Categories
Funding
- NIH [P41GM103832, HL071895, HL085769, HL081613, CA177909]
- Department of Defense [OC120547, OC093416]
- Ovarian Cancer Research Fund Program Project Development Grant
- Bettyann Asche Murray Distinguished Professorship
- Baylor College of Medicine
- MD Anderson Cancer Center Collaborative Award
Ask authors/readers for more resources
Thrombocytosis and platelet hyperreactivity are known to be associated with malignancy; however, there have been no ultrastructure studies of platelets from patients with ovarian cancer. Here, we used electron cryotomography (cryo-ET) to examine frozen-hydrated platelets from patients with invasive ovarian cancer (n = 12) and control subjects either with benign adnexal mass (n = 5) or free from disease (n = 6). Qualitative inspections of the tomograms indicate significant morphological differences between the cancer and control platelets, including disruption of the microtubule marginal band. Quantitative analysis of subcellular features in 120 platelet electron tomograms from these two groups showed statistically significant differences in mitochondria, as well as microtubules. These structural variations in the platelets from the patients with cancer may be correlated with the altered platelet functions associated with malignancy. Cryo-ET of platelets shows potential as a noninvasive biomarker technology for ovarian cancer and other platelet-related diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available