Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 50, Pages E6907-E6916Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1507833112
Keywords
DNA repair; genome stability; translocation
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Funding
- NIH [GM32431, GM58015]
- California Breast Cancer Research Program (CBCRP) Fellowship [15IB-0109]
- Training Grant in Oncogenic Signals and Chromosome Biology [CA108459]
- Presidential Undergraduate Fellowship from the University of California
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Maintenance of genome stability is carried out by a suite of DNA repair pathways that ensure the repair of damaged DNA and faithful replication of the genome. Of particular importance are the repair pathways, which respond to DNA double-strand breaks (DSBs), and how the efficiency of repair is influenced by sequence homology. In this study, we developed a genetic assay in diploid Saccharomyces cerevisiae cells to analyze DSBs requiring microhomologies for repair, known as microhomology-mediated end-joining (MMEJ). MMEJ repair efficiency increased concomitant with microhomology length and decreased upon introduction of mismatches. The central proteins in homologous recombination (HR), Rad52 and Rad51, suppressed MMEJ in this system, suggesting a competition between HR and MMEJ for the repair of a DSB. Importantly, we found that DNA polymerase delta (Pol delta) is critical for MMEJ, independent of microhomology length and base-pairing continuity. MMEJ recombinants showed evidence that Pol delta proofreading function is active during MMEJ-mediated DSB repair. Furthermore, mutations in Pol delta and DNA polymerase 4 (Pol the DNA polymerase previously implicated in MMEJ, cause a synergistic decrease in MMEJ repair. Pol lambda, showed faster kinetics associating with MMEJ substrates following DSB induction than Pol delta. The association of Pol delta depended on RAD1, which encodes the flap endonuclease needed to cleave MMEJ intermediates before DNA synthesis. Moreover, Pol delta recruitment was diminished in cells lacking Pol lambda. These data suggest cooperative involvement of both polymerases in MMEJ.
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