Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 10, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1479-5876-10-69
Keywords
Human; T Cells; Tumor Immunity; T Cell Receptors; Adoptive Immunotherapy
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Funding
- National Cancer Institute
- Adelson Medical Research Foundation
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Background: To simplify clinical scale lymphocyte expansions, we investigated the use of the WAVE (R), a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes. Methods: We have developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant numbers of cells for our adoptive cell transfer clinical protocols. Results: TIL and genetically modified PBL were rapidly expanded to clinically relevant scales in both static bags and the WAVE bioreactor. Both bioreactors produced comparable numbers of cells; however the cultures generated in the WAVE bioreactor had a higher percentage of CD4+ cells and had a less activated phenotype. Conclusions: The WAVE bioreactor simplifies the process of rapidly expanding tumor reactive lymphocytes under GMP conditions, and provides an alternate approach to cell generation for ACT protocols.
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