Use of ion chromatography for the determination of selected metals in blood serum of patients with type 2 diabetes

Ion chromatography followed by microwave-induced acid digestion was used to evaluate the serum levels of Fe3+, Cu2+, Ni2+ Zn2+, and Mn2+ in patients with diagnosed type 2 diabetes and in healthy controls. Recoveries ranged from 98.0% to 102% for Fe3+, from 89.9% to 100% for Cu2+, from 87.9% to 102% for Zn2+, and from 89.6% to 102% for Mn2+ were determined by examining samples spiked with various amounts of all the studied ions. The time of mineralization longer than 28 min did not affect the assay values. Precision was assessed at four unique concentrations in replicates of six, on four separate occasions. RSD was determined to be 1.16% for Fe3+, 5.20% for Cu2+, 2.8% for Zn2+, and 3.75% for Mn2+. The accuracy results (values of RSD) were as follows: 5.16% for Fe3+ 6.35% for Cu2+, 4.9% for Zn2+, and 7.23% for Mn2+. The statistical analysis confirmed that mean concentrations of Fe3+ and Zn2+ did not differ significantly from analogous values in the control group. Patients who additionally suffered from hypertension had higher copper concentrations compared with diabetic patients. For diabetics the presence of Mn2+ was not stated (LOD values amounting to 0.006 mu g/mL). Ni2+ was not detectable for either the studied group or the control group (LOD = 0.006 mu g/mL). (C) 2009 Elsevier GmbH. All rights reserved.