Journal
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume 12, Issue 10, Pages 2088-2098Publisher
WILEY
DOI: 10.1002/term.2743
Keywords
endometrial mesenchymal stem cells; extracellular vesicles; immunomodulation; lymphocyte activation; lymphocyte differentiation; TGF
Categories
Funding
- ERDF/ESF [CP17/00021]
- Consejeria de Economia e Infraestructuras, Junta de Extremadura
- FEDER [IB16168]
- GobEx (Ayuda a grupos catalogados de la Junta de Extremadura) [GR15175]
- Juan de la Cierva Incorporacion from the Spanish Ministry of Economy, Industry and Competitiveness [IJCI-2014-19428]
- Spanish Ministry of Economy and Competitiveness (MINECO) [BIO2015-67580-P]
- Carlos III Institute of Health-Fondo de Investigacion Sanitaria [PT13/0001/0017-ISCIII-SGEFI/FEDER, PT17/0019/0003 ISCIII-SGEFI/FEDER]
- ProteoRed
- Fundacion La Marato TV3 [20153731(122/C/2015)]
- CIBERCV [CB16/11/00494, CB16/11/00277]
- Ministerio de Ciencia, Innovacion y Universidades
- Pro-CNIC Foundation
Ask authors/readers for more resources
Endometrial mesenchymal stem cells (endMSCs) reside in the basal and functional layer of human endometrium and participate in tissue remodelling, which is required for maintaining the regenerative capacity of the endometrium. The endMSCs are multipotent stem cells and exhibit immunomodulatory effects. This paper aimed to evaluate the regulatory effects of extracellular vesicles derived from endMSCs (EV-endMSCs) in the setting of T cell activation. In vitro stimulations of lymphocytes were performed in the presence of EV-endMSCs. These in vitro-stimulated lymphocytes were functionally and phenotypically characterized to distinguish CD4+ and CD8+ T cell differentiation subsets. Moreover, the inhibition of TGF was performed with neutralizing antibodies. The phenotype and nanoparticle tracking analysis of the EV-endMSCs demonstrated that they are similar in terms of size distribution to other mesenchymal stem cells-derived exosomes. The in vitro assays showed an immunomodulatory potential of these vesicles to counteract the differentiation of CD4+ T cells. The quantification of active TGF in EV-endMSCs was found to be very high when compared with extracellular vesicles-free concentrated supernatants. Finally, the neutralization of TGF significantly attenuated the immunomodulatory activity of EV-endMSCs. In summary, this is the first report demonstrating that EV-endMSCs exhibit a potent inhibitory effect against CD4+ T cell activation, which is partially mediated by TGF signalling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available