Journal
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
Volume 9, Issue 2, Pages 333-338Publisher
WILEY
DOI: 10.1111/j.1538-7836.2010.04152.x
Keywords
clearance; hemophilia patients; modeling; NONMEM; pharmacokinetics; rFVIIa
Categories
Funding
- Novo Nordisk A/S, Bagsvaerd, Denmark
- General Clinical Research Center [M01 RR00065]
- NCR
- National Institutes of Health
Ask authors/readers for more resources
Objective: The objective of the present study was to evaluate the pharmacokinetics and the clearance pathways of rFVIIa after intravenous administration to hemophilia patients. Methods: Ten severe hemophilia patients were included in the study; all patients were intravenously administered a clinically relevant dose of 90 mu g kg-1 (1.8 nmol kg-1) rFVIIa. Blood samples were collected consecutively to describe the pharmacokinetics of rFVIIa. All samples were analyzed using three different assays: a clot assay to measure the activity (FVIIa:C), an enzyme immunoassay (EIA) to measure the antigen levels (FVII:Ag), and an EIA (FVIIa-AT) to measure the FVIIa antithrombin III (AT) complex. Pharmacokinetic parameters were evaluated both by use of standard non-compartmental methods and by use of mixed effects methods. A population pharmacokinetic model was used to simultaneously model all three datasets. The total body clearance of rFVIIa:C was estimated to be 38 mL h-1 kg-1. The rFVII-AT complex formation was responsible for 65% of the total rFVIIa:C clearance. The initial and the terminal half-life of rFVIIa:C was estimated to be 0.6 and 2.6 h, respectively. The formation of rFVII-AT complex was able to explain the difference observed between the rFVIIa:C and the rFVII:Ag concentration. The non-compartmental analysis resulted in almost identical parameters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available