Prediction of optimum process parameters to achieve eco-friendly desizing of organic cotton fabrics with indigenously produced alpha-amylase and their enzyme kinetics

An attempt has been made to study the desizing of polyvinyl alcohol (PVA) starch-based organic cotton fabric using alpha-amylase enzyme with various process parameters such as enzyme concentration, temperature and reaction time. These process variables are selected based on the Box-Behnken design of experiment and the output of experiment resulted in weight loss of the fabric and their results are optimized with minimum error. The test results are analyzed using Design-Expert software 8.0 to predict the optimum process parameters to achieve the required desizing efficiency and their results are compared with actual trials. The alpha-amylase enzyme kinetics are studied at various concentrations, temperatures and reaction times to optimize the desizing process parameters. The performance of desizing was assessed by an iodine test and FTIR results to confirm the degradation of PVA starch in the desized organic cotton fabrics.