Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 93, Issue 15, Pages 3870-3875Publisher
WILEY
DOI: 10.1002/jsfa.6342
Keywords
disease resistance; embryo rescue; grapevine; seedless grapes; stenospermocarpy
Funding
- Project of Special Fund for Agro-scientific Research in the Public Interest [200903044-4]
- earmarked fund for China Agriculture Research System [CARS-30-yz-7]
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BACKGROUNDSince 1982, the embryo rescue technique has been widely applied to embryo germination of stenospermic grapes in cross-breeding programmes. This project aimed to: (1) use embryos to breed new seedless cultivars of Vitis vinifera as the female parents utilising wild Chinese Vitis spp. as the male parents; and (2) develop an efficient method for in vitro embryo rescue and plant development. RESULTSAmong the different genotypes, the productions of hybrid plants were significantly different, ranged from 21.1% (Ruby Seedless' x Beichun') to only 1.1% (Pink Seedless' x Beichun'), except for the combinations from which no surviving seedlings were obtained. We collected hybridisation fruits from 28 June to 3 August, and obtained their best sampling times described within days after flowering. The highest rates of embryo formation (24.3%) and plant development (91.4%) were found when ovules of Ruby Seedless' were cultured in MM4 + 500 mg L-1 mashed banana. CONCLUSIONSeven new hybrids of V. vinifera with wild Chinese Vitis spp. were obtained. As a result of early nuclear-free character identification, 17 seedless grape lines were obtained. An efficient system of seedless grape breeding through embryo rescue was also established. (c) 2013 Society of Chemical Industry
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