Journal
JOURNAL OF THE ROYAL SOCIETY INTERFACE
Volume 11, Issue 95, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rsif.2014.0097
Keywords
allergic inflammation; oxidative stress; pollen; static magnetic field
Categories
Funding
- Hungarian Scientific Research Fund [NK-101538, K-73347, K-109595]
- European Union
- State of Hungary
- European Social Fund [TAMOP 4.2.4.A/2-11-1-2012-0001, TAMOP 4.2.2/B-10/1-2010-0024, TAMOP-4.2.2.A-11/1/KONV-2012-0023, TAMOP-4.2.2.C-11/1/KONV-2012-0001]
- Janos Bolyai Fellowship from the Hungarian Academy of Sciences
- Faculty of Medicine Research Fund from the University of Debrecen
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Previous observations suggest that static magnetic field (SMF)-exposure acts on living organisms partly through reactive oxygen species (ROS) reactions. In this study, we aimed to define the impact of SMF-exposure on ragweed pollen extract (RWPE)-induced allergic inflammation closely associated with oxidative stress. Inhomogeneous SMF was generated with an apparatus validated previously providing a peak-to-peak magnetic induction of the dominant SMF component 389 mT by 39 T m(-1) lateral gradient in the in vivo and in vitro experiments, and 192 mT by 19 T m(-1) in the human study at the 3 mm target distance. Effects of SMF-exposure were studied in a murine model of allergic inflammation and also in human provoked skin allergy. We found that even a single 30-min exposure of mice to SMF immediately following intranasal RWPE challenge significantly lowered the increase in the total antioxidant capacity of the airways and decreased allergic inflammation. Repeated (on 3 consecutive days) or prolonged (60 min) exposure to SMF after RWPE challenge decreased the severity of allergic responses more efficiently than a single 30-min treatment. SMF-exposure did not alter ROS production by RWPE under cell-free conditions, while diminished RWPE-induced increase in the ROS levels in A549 epithelial cells. Results of the human skin prick tests indicated that SMF-exposure had no significant direct effect on provoked mast cell degranulation. The observed beneficial effects of SMF are likely owing to the mobilization of cellular ROS-eliminating mechanisms rather than direct modulation of ROS production by pollen NAD(P)H oxidases.
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