Journal
JOURNAL OF THE ROYAL SOCIETY INTERFACE
Volume 8, Issue 61, Pages 1204-1211Publisher
ROYAL SOC
DOI: 10.1098/rsif.2010.0747
Keywords
plasmonics; antibody microarray; nanoparticle labelling
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Funding
- European commission [22364]
- EPSRC [DT/E010857/1]
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The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV-visible absorption spectroscopy. A value of 1.3 x 10(7) M(-1) was obtained for the antibody-antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.
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