Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 46, Issue 2, Pages 171-175Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2015.1015561
Keywords
A40926; fermentation; lipoglycopeptide antibiotic; L-leucine; medium optimization; Nonomuraea sp; DP-13
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Funding
- Liaoning Province Department of Education Science Research [L2013215]
- Liaoning Provincial Natural Science Foundation [2014026011]
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The lipoglycopeptide antibiotic A40926 produced by Nonomuraea sp. is a complex of structurally related components differing in the fatty acid moiety. Besides showing an intrinsic antibacterial activity, A40926 is the precursor of the semisynthetic antibiotic Dalvance. In this work, A40926 production by a mutant strain Nonomuraea sp. DP-13 was investigated. It was found that A40926 production was markedly promoted by using poorly assimilated carbon source maltodextrin and nitrogen source soybean meal. Addition of Cu2+ resulted in a stimulation of A40926 production, but Co2+ had an inhibitory effect. L-Leucine addition greatly improved total A40926 production and modified the complex composition toward factor B0. An optimized production medium IM-3 was developed and a maximum A40926 production of 1096mg/L was obtained in the 10-L fermenter. This was the highest A40926 productivity so far reported.
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