Journal
JOURNAL OF THE NEUROLOGICAL SCIENCES
Volume 329, Issue 1-2, Pages 38-44Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jns.2013.03.011
Keywords
Stanniocalcin-1; Neural cells; Mitochondrial membrane potential; Hormesis; Stress; Neurodegeneration
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Funding
- Sigrid Juselius Foundation
- Finska Lakaresallskapet
- Magnus Ehrnrooth Foundation
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We induced upregulation of stanniocalcin-1 (STC-1) by various mild and long lasting stresses and assayed its influence on mitochondrial membrane potential (MMP) in the neural crest-derived cell line Paju. The obtained data showed that starvation (24-96 h), exposure to 10 nM TPA, and low concentrations (0.05-1 mu M) of As2O3 significantly (3-5 times) upregulated Paju cell STC-1 RNA and stabilized the mitochondrial membrane potential (MMP). However, high concentrations of As2O3 (2.5-5.0 mu M) increased intracellular ROS and free calcium levels and, consequently, suppressed STC-1 and MMP. The results show that cells preconditioned by various mild stresses expressed more STC-1 and their MMP were more resistant to a secondary exposure to As2O3 (2.5-5 mu M, 96 h) demonstrating mitohormesis. We suggest that MMP deviation from control levels, to an extent innocuous to cell viability, is a general signal for STC-1-induction and MMP-protection. Our findings of Paju cell MMP-regulation may be of great importance for inventing new ways to prevent neurodegenerative diseases and unravel the mechanisms behind drug resistance. (C) 2013 Elsevier B.V. All rights reserved.
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