Journal
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
Volume 29, Issue -, Pages 225-234Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmbbm.2013.09.002
Keywords
Synthetic spider silk fibers; Mechanical properties; Post-spinning; Dragline; Argiope aurantia
Funding
- National Institute of Health (NIH) [EB000490]
- Department of Energy (DOE) [DE-SC0004791]
- Department of Defense Air Force Office of Scientific Research (AFOSR) [FA9550-09-1-0717]
- Department of Defense AFOSR [FA9550-10-1-0275]
- National Science Foundation (NSF), Division of Materials Research [DMR-0805197]
- U.S. Department of Energy, Basic Energy Sciences, Office of Science [DE-ACO206CH11357]
- National Center for Research Resources [5P41RR007707]
- National Institute of General Medical Sciences from the National Institutes of Health [8P41GM103543]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1264801] Funding Source: National Science Foundation
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Spider silk is a biomaterial with impressive mechanical properties, resulting in various potential applications. Recent research has focused on producing synthetic spider silk fibers with the same mechanical properties as the native fibers. For this study, three proteins based on the Argiope aurantia Major ampullate Spidroin 2 consensus repeat sequence were expressed, purified and spun into fibers. A number of post-spin draw conditions were tested to determine the effect of each condition on the mechanical properties of the fiber. In all cases, post-spin stretching improved the mechanical properties of the fibers. Aqueous isopropanol was the most effective solution for increasing extensibility, while other solutions worked best for each fiber type for increasing tensile strength. The strain values of the stretched fibers correlated with the length of the proline-rich protein sequence. Structural analysis, including X-ray diffraction and Raman spectroscopy, showed surprisingly little change in the initial as-spun fibers compared with the post-spin stretched fibers. (C) 2013 Elsevier Ltd. All rights reserved.
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