Determination of furazolidone in urine by square-wave voltammetric method

A fast, simple and sensitive square-wave voltammetric (SWV) method for the determination of trace amounts of furazolidone (FZ) in urine is reported. A three-electrode system containing stationary mercury dropping (SMDE) working electrode, Pt auxiliary electrode and Ag/AgCl reference electrode was used throughout. Briton-Rabinson buffer solution is used as both pH adjusting agent and supporting electrolyte. The calibration graph showed good linearity in the concentration range of 20-900 ng ml(-1) of furazolidone with a regression coefficient of 0.9996. The equation Delta(i) = 0.0095C(FZ) + 0.234 was used for calculation of furazolidone concentration in the sample solution, where C-FZ is the concentration of furazolidone in ng ml(-1) and A(i) is the difference between voltammogram peak currents of sample and blank solution. The RSD for 8 replicate measurements of a 60 ng ml-1 solution and LOD of the proposed method were found to be 2.2% and 5.2 ng ml(-1), respectively. The procedure was successfully applied to the determination of furazolidone in urine samples.