Aneuploidy, copy number variation and unique chromosomal structures in bottom-fermenting yeast revealed by array-CGH

DNA microarray for comparative genome hybridization (CGH) of bottom-fermenting yeast was performed based on our in-house DNA sequence data. Aneuploidy, copy number variation and unique chromosomal structures were observed among bottom-fermenting yeast strains. Our array experiments revealed a correlation between copy number variation and mRNA expression levels. Chromosomal structures in a Saccharomyces carlsbergensis-type strain and in a S. monacensis-type strain that both belong to S. pastorianus phylogenetically differed greatly from those in contemporary industrial bottom-fermenting yeast strains. The knowledge gained in this study contributes to a more precise genomic characterization of bottom-fermenting yeast strains. Copyright (c) 2014 The Institute of Brewing & Distilling