Journal
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
Volume 49, Issue 2, Pages 82-87Publisher
FOOD HYG SOC JPN
DOI: 10.3358/shokueishi.49.82
Keywords
heat resistant mould; Byssochlamys; Talaromyces; Thermoascus; Paecilomyces; lys2; 18s rRNA; 18S rRNA gene; 26/28s rRNA D2 26/28S rRNA gene D2 region; identification; molecular phylogenetic analysis
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Comparative sequence analysis was performed on the 18S rRNA gene (1,676 bp), 26/28S rRNA gene D2 region (321 bp) and lys2 (997 bp) to evaluate the gene index for rapid, accurate and convenient identification of Byssochlamys spp. and related species. The results showed that 26 strains (11 species) of the clade could be identified or grouped by means of each gene sequence. The highest resolution to discriminate these species was observed with lys2, but 26/28S rRNA gene D2 region was considered to be the best index for convenient identification. In addition, phylogenetic analysis based on these genes indicated that genus Byssochlamys is not monophyletic, although species in the clade are closely related to each other. Re-classification will be necessary, based on detailed morphological observations.
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