Journal
JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS
Volume 3, Issue -, Pages -Publisher
EUROPEAN OPTICAL SOC
DOI: 10.2971/jeos.2008.08034
Keywords
optical trapping and manipulation; coherent anti-Stokes Raman scattering; fluorescence spectroscopy; optical systems
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Funding
- Carlsberg Foundation
- Danish Agency for Science, Technology and Innovation
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Optical trapping has enabled a multitude of applications focusing, in particular, on non-invasive studies of cellular material. The full potential of optical trapping has, however, not yet been exploited due to restricted access to the trapped samples, caused by high numerical aperture objectives needed to focus the trapping laser beams. Here, we use our recently developed biophotonics workstation to overcome this limitation by introducing probing and spectroscopic characterization of optically trapped particles in a side-view geometry perpendicular to the trapping beams rather than in the traditional top-view geometry parallel to the trapping beams. Our method is illustrated by CARS and fluorescence spectroscopy of trapped polystyrene beads. The side-view geometry opens intriguing possibilities for accessing trapped particles with optical as well as other types of probe methods independent from the trapping process. [DOI: 10.2971/jeos.2008.08034]
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