Journal
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
Volume 157, Issue 10, Pages J334-J337Publisher
ELECTROCHEMICAL SOC INC
DOI: 10.1149/1.3478635
Keywords
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Funding
- U.S. Army [W911NF-05-1-0339]
- Center for Advanced Materials Processing (CAMP) at Clarkson University
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Peanut protein Ara h 1, a common food allergen, has been previously detected at antibody-coated Au electrodes using electrochemical impedance spectroscopy (EIS) in ideal solutions, free from interfering species. Here, peanut protein Ara h 1 is detected by EIS at antibody-coated Au electrodes in canned soup that is filtered, diluted, and spiked with peanut protein Ara h 1. In this system, the combined strategy of sample dilution and blocking of unreacted surface sites on the Au electrode with bovine serum albumin is sufficient to dramatically reduce nonspecific adsorption. This is demonstrated by a quantitative comparison of the impedance change at two Au electrodes, one coated with the antibody to peanut protein Ara h 1 and the other coated with the antibody to cockroach protein Bla g 1. These results suggest that nonspecific adsorption does not in general limit the utility of biosensors based on EIS. (C) 2010 The Electrochemical Society. [DOI: 10.1149/1.3478635] All rights reserved.
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