Simultaneous Determination of Hesperidin, Ferulic Acid, Cinnamic Acid and Cinnamaldehyde in Chinese Tonic Wine by High Performance Liquid Chromatography

This study aims to establish a H PLC method for the simultaneous determination of hesperidin (HE), ferulic acid (FA), cinnamic acid (CA) and cinnamaldehyde (CAD) in a Chinese tonic wine, Ma-Zu-Wan-Show-Yao-Jyo. After filtering the tonic wine, the filtrate was separated on a C18 column (250 x 4.6 mm, 5 mu m) by gradient elution of acetonitrile-aqueous solution The regression equations were linear (r >= 0.9999) in the range of 1.5-150.0 mu g/mL for HE, 0.3-60.0 mu g/mL for FA, 0.06-6.0 mu g/mL for CA, and 0.1-80 0 mu g/mL for CAD. The relative standard deviation and relative error were less than 5 0% and 4.0%, respectively, for intra- and inter-day analyses. The limits of detection (LOD) were 0.6, 0 075, 0.012 and 0.02 mu g/mL for HE, FA, CA and CAD (S/N = 3, 20 mu l injection) respectively This method can be used to standardize the quality control of Chinese tonic wine to ensure accuracy and efficiency in the future