Polar Constituents from Sageretia thea Leaf Characterized by HPLC-SPE-NMR Assisted Approaches

Nine glycosides (1-9) were characterized from the n-butanol-soluble fraction of the ethanolic extract of the leaves of Sageretia thea by the general approach. Among these, Compounds 6 and 7 were identified as a mixture. Application of HPLC-SPE-NMR in two selected fractions led to the separation of this mixture and the characterization of three additional minors (10-12). Among these, 7-O-methylmyricetin 3-O-alpha-L-arabinofuranoside (8) is a new natural product and eight compounds, i.e. glucofragulin A (1), quercetin-3-O-alpha-L-arabinopyranoside (5), 3-O-beta-D-galactopyranoside (6), 3-O-beta-D-glucopyranoside (7), and 3-O-alpha-L-arabinofuranoside (11), myricetin-3-O-alpha-L-arabinofuranoside (9) and 3-O-beta-D-glucopyranoside (10), and quercetrin (12), are found for the first time from the title plant.