Journal
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
Volume 20, Issue 12, Pages 2593-2603Publisher
AMER SOC NEPHROLOGY
DOI: 10.1681/ASN.2009020132
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Funding
- European Community [FP7/2007-2013]
- European Research Council (ERC) [223007, 205027]
- Dutch Kidney Foundation [NSN 06.2191]
- Tuscany Ministry of Health
- Fondazione Cariplo, Milan, Italy [2007-5549]
- Associazione Italiana per la Ricerca sul Cancro
- Federazione Italiana per la Ricerca sul Cancro
- Collegio Ghislieri
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Glomerular injury can involve excessive proliferation of glomerular epithelial cells, resulting in crescent formation and obliteration of Bowman's space. The origin of these hyperplastic epithelial cells in different glomerular disorders is controversial. Renal progenitors localized to the inner surface of Bowman's capsule can regenerate podocytes, but whether dysregulated proliferation of these progenitors contributes to crescent formation is unknown. In this study, we used confocal microscopy, laser capture microdissection, and real-time quantitative reverse transcriptase-PCR to demonstrate that hypercellular lesions of different podocytopathies and crescentic glomerulonephritis consist of three distinct populations: CD133(+)CD24(+) podocalyxin (PDX)(-)nestin(-) renal progenitors, CD133(+)CD24(+) PDX(+)nestin(+) transitional cells, and CD133(-)CD24(-)PDX(+)nestin(+) differentiated podocytes. In addition, TGF-beta induced CD133(+)CD24(+) progenitors to produce extracellular matrix, and these were the only cells to express the proliferation marker Ki67. Taken together, these results suggest that glomerular hyperplastic lesions derive from the proliferation of renal progenitors at different stages of their differentiation toward mature podocytes, providing an explanation for the pathogenesis of hyperplastic lesions in podocytopathies and crescentic glomerulonephritis.
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