Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 25, Issue 2, Pages 269-277Publisher
SPRINGER
DOI: 10.1007/s13361-013-0782-y
Keywords
Mass spectrometry; Nanodisc; Membrane proteins
Funding
- Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center
- US Department of Energy, Office of Basic Energy Science [DE-SC 0001035]
- National Institute of General Medical Sciences [8 P41 GM103422-35]
- National Institute of Health [R01-GM31756, R01-GM33775]
- NSF [IBDR 0964199]
- Robert C. and Carolyn J. Springborn Graduate Fellowship
- DOE
- NIGMS
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Nanodiscs are a promising system for studying gas-phase and solution complexes of membrane proteins and lipids. We previously demonstrated that native electrospray ionization allows mass spectral analysis of intact Nanodisc complexes at single lipid resolution. This report details an improved theoretical framework for interpreting and deconvoluting native mass spectra of Nanodisc lipoprotein complexes. In addition to the intrinsic lipid count and charge distributions, Nanodisc mass spectra are significantly shaped by constructive overlap of adjacent charge states at integer multiples of the lipid mass. We describe the mathematical basis for this effect and develop a probability-based algorithm to deconvolute the underlying mass and charge distributions. The probability-based deconvolution algorithm is applied to a series of dimyristoylphosphatidylcholine Nanodisc native mass spectra and used to provide a quantitative picture of the lipid loss in gas-phase fragmentation.
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