Fast, High Resolution Mass Spectrometry Imaging Using a Medipix Pixelated Detector

In mass spectrometry imaging, spatial resolution is pushed to its limits with the use of ion microscope mass spectrometric imaging systems An ion microscope magnifies and then projects the original spatial distribution of ions from a sample surface onto a position-sensitive detector, while retaining time-of-flight mass separation capabilities Here, a new type of position-sensitive detector based on a chevron microchannel plate stack in combination with a 512 x 512 complementary metal-oxide-semiconductor based pixel detector is coupled to an ion microscope Spatial resolving power better than 6 mu m is demonstrated by secondary ion mass spectrometry and 8-10 mu M spatial resolving power is achieved with laser desorption ionization A detailed evaluation of key performance criteria such as spatial resolution, acquisition speed, and data handling is presented (J Am Soc Mass Spectrom 2010, 21, 2023-2030) (C) 2010 Published by Elsevier Inc on behalf of American Society for Mass Spectrometry