Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 20, Issue 11, Pages 2006-2012Publisher
SPRINGER
DOI: 10.1016/j.jasms.2009.07.019
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Funding
- NCI NIH HHS [R33 CA105295-04, R33 CA105295] Funding Source: Medline
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PC-IDMS experiments for two peptides, laminin nonapeptide and the N-terminal tryptic peptide of prostate specific antigen, were performed utilizing a variety of alkylating reagents. These experiments were conducted to investigate how hydrophobicity influences the limits-of-detection (LOD) by altering their electrospray ionization response. Nonpolar surface areas were calculated for both peptides and all alkylating reagents to provide an estimate of the hydrophobicity of the differently alkylated peptides. Decreases in LOD by 2-fold were observed for both peptides between the best and worst performing combination of alkylating reagent. However, while an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophobicity, we observed a decrease. (J Am Soc Mass Spectrom 2009, 20, 2006-2012) (C) 2009 American Society for Mass Spectrometry
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