Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 19, Issue 4, Pages 560-568Publisher
AMER CHEMICAL SOC
DOI: 10.1016/j.jasms.2008.01.008
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The interaction of copper ions with peptides was investigated by electrospray mass spectrometry. Two electrospray micro-emitters were compared, the first one with a platinum electrode using a copper(II) electrolyte solution containing a peptide sample, and the second one with a sacrificial copper anode in a water/methanol solution containing only a peptide (i.e., angiotensin III, bradykinin, or Leu-enkephalin). The former yielded mainly Cu2+ complexes either with histidine residues or with the peptide backbone (Cu+ complexes can be also formed due to gas-phase reactions), whereas the latter can generate a mixture of both Cu+ and Cu2+ aqueous complexes that yield different complexation patterns. This study shows that electrospray emitters with soluble copper anodes enable the study of Cu(l)-peptide complexes in solution.
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