Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 19, Issue 1, Pages 82-90Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jasms.2007.10.012
Keywords
-
Funding
- NCRR NIH HHS [R41 RR023764, R41 RR023764-01] Funding Source: Medline
- NHLBI NIH HHS [R01 HL062244-05A1, R01 HL062244, R01 HL062244-06, HL62244] Funding Source: Medline
- NIGMS NIH HHS [R01 GM038060-19, R01 GM038060-18, R01 GM038060, GM38060] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [R41RR023764] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL062244] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM038060] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1-->3 and in NAH 1-->4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions (0,2)A(n) and (0,2)A(n)-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an (0,2)A(2) ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available