4.3 Article

Optimization of Physiological Growth Conditions for Maximal Gamma-linolenic Acid Production by Cunninghamella blakesleeana-JSK2

Journal

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 91, Issue 9, Pages 1507-1513

Publisher

WILEY
DOI: 10.1007/s11746-014-2507-1

Keywords

Gamma-linolenic acid; Submerged fermentation; Cunninghamella blakesleeana-JSK2

Funding

  1. University Grant Council (UGC), India
  2. Grant Agency of the Ministry of Education, Slovak Republic [VEGA 1/0975/12]
  3. Slovak Research and Development Agency, Slovak Republic [APVV-0662-11, APVV-0294-11]

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Effects of various cultural conditions on biomass, lipid and Gamma-linolenic acid (GLA) production were investigated in the oleaginous fungus Cunninghamella blakesleeana-JSK2 isolated from soil. The GLA production was influenced by various factors such as growth condition (static and shaken), incubation time, pH, temperature, carbon and nitrogen sources. The results indicated that optimum GLA production (21 %) was obtained when the fungus was grown under shaken condition at 120 rpm for 6 days with optimum pH and temperature of 6 and 28 A degrees C ,respectively. Glucose and potassium nitrate enhanced the GLA production. Urea and sucrose were poor substances for biomass, lipid and GLA production.

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