Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 90, Issue 8, Pages 1131-1137Publisher
WILEY
DOI: 10.1007/s11746-013-2231-2
Keywords
Castor oil; Enzymatic hydrolysis; gamma-Decalactone; Lipase; Yarrowia lipolytica
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Funding
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BD/28039/2006, SFRH/BD/63701/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/63701/2009, SFRH/BD/28039/2006] Funding Source: FCT
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gamma-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the gamma-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L-1 was found to be the optimal oil concentration. Under these conditions, cells were able to produce lipase but at low activity levels, which might limit ricinoleic acid release and consequently, the gamma-decalactone production rate. Thus, the enzymatic hydrolysis of CO by commercial lipases was studied under different operating conditions. Lipozyme TL IM was found to be the most efficient and the optimal hydrolysis conditions were pH 8 and 27 A degrees C. The use of hydrolyzed CO in the aroma production allowed a decrease in the lag phase for gamma-decalactone secretion.
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