Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 89, Issue 2, Pages 189-198Publisher
WILEY
DOI: 10.1007/s11746-011-1903-z
Keywords
Algae; Lipid extraction; Solvent mixture; Pretreatment; Cell disruption; Fat content
Categories
Funding
- Institute for the Promotion of Innovation by Science and Technology-Strategic Basic Research (IWT-SBO)
- K.U. Leuven Kulak
Ask authors/readers for more resources
An optimized procedure for extraction of total and non-polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell-disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform-methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell-disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non-polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available