Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 88, Issue 4, Pages 473-480Publisher
WILEY
DOI: 10.1007/s11746-010-1702-y
Keywords
Acidolysis; Capric acid; Caprylic acid; Olive oil; Rhizopus oryzae lipase; Structured lipids
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [SFRH/BD/39280/2007]
- CYTED
- Spanish Ministry of Science and Innovation [CTQ2007-60347]
- Reference Network in Biotechnology (XRB) (Generalitat de Catalunya) [2009-SGR-281]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/39280/2007] Funding Source: FCT
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This work aims to produce triacylglycerols (TAG) containing a medium-chain fatty acid (M) at positions sn-1,3 and a long-chain fatty acid (L) at sn-2 position, i.e. TAG of MLM type, by acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, catalyzed by 1,3-selective Rhizopus oryzae heterologous lipase (rROL) immobilized in Eupergit (R) C and modified sepiolite. This lipase was produced by the methylotrophic yeast Pichia pastoris. Reactions were performed at 25 and 40 degrees C, for 24 h, either in solvent-free or in n-hexane media, at a molar ratio 1:2 (olive oil:free fatty acid). Higher incorporations of C8:0 (21.6 mol%) and C10:0 (34.8 mol%) into the TAG were attained in solvent-free media, at 40 degrees C, when rROL immobilized in Eupergit (R) C was used. In organic media, at 40 degrees C, only 15.9 and 14.1 mol%, incorporation of C8:0 or C10:0 were, respectively observed. Lower incorporations were attained for both acids (3.4-7.0 mol%) when native ROL (nROL) in both supports and rROL in modified sepiolite were used. rROL in Eupergit (R) C maintained its activity during the first four or three 23-h batches, respectively when C8:0 (half-life time, t(1/2) = 159 h) or C10:0 (t(1/2) = 136 h) were used, decreasing thereafter following a time delay model.
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