Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 86, Issue 8, Pages 757-763Publisher
SPRINGER
DOI: 10.1007/s11746-009-1403-6
Keywords
P-31 NMR; TLC; Dairy phospholipids
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Funding
- New Zealand Foundation for Research, Science and Technology [DRIX0302]
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P-31 NMR analysis of samples prepared in a sodium cholate detergent system was assessed as a method for the quantitative measurement of dairy phospholipids. Major phospholipid (PL) classes measured included: phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SM) and dihydrosphingomyelin (DHSM). The P-31 NMR method was validated by comparison with a quantitative two-dimensional thin-layer chromatography (2D-TLC) technique. The 2D-TLC system was more sensitive, able to detect some minor compounds not observed by P-31 NMR. However, P-31 NMR is more suited to routine analysis, with sample analysis taking 36 min. The method was also more versatile and sample analysis was possible on high phospholipid containing materials without prior lipid extraction (e.g. buttermilk protein concentrate, beta serum liquid).
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