Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 87, Issue 2, Pages 147-155Publisher
WILEY
DOI: 10.1007/s11746-009-1486-0
Keywords
Sinapic acid derivatives; Canola extracts; Liquid chromatography
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canola Council of Canada
- Syngenta Inc, Canada
Ask authors/readers for more resources
A high-performance liquid chromatographic (HPLC) method with diode array detection (DAD) was used to determine the total phenolics, including sinapic acid derivatives in canola. Ten Western Canadian canola seeds, six other commodity canola seeds, their corresponding press cakes and meals were analyzed. Seeds of European 00 rapeseed and Brassica Juncea (Indian mustard) were included for comparison. Phenolic compounds were separated using a gradient elution system of water-methanol-omicron-phosphoric acid solution with a flow rate of 0.8 ml/min. In addition to sinapine (SP) and sinapic acid (SA), sinapoyl glucose (SG) is reported in the methanolic extracts. The detection and quantification limits of these compounds were 0.20-0.40 and 0.50-0.80 mu g/ml, respectively with recovery values over 98.0%. The content of total phenolics, SP, SA and SG in canola extracts ranged from 9.16 to 16.13, 6.39 to 12.28, 0.11 to 0.59 and 1.36 to 7.50 mg/g, respectively with significant differences among varieties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available