Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 85, Issue 8, Pages 739-748Publisher
WILEY
DOI: 10.1007/s11746-008-1261-7
Keywords
Phospholipase A1; immobilization; gelatin; rice bran oil; degumming; spinning basket reactor
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Immobilization of Lecitase (Phospholipase A1) in gelatin hydrogel and its stability is studied with a view to utilizing the immobilized enzyme for degumming rice bran oil. Excellent retention of enzyme activity (> 80%) is observed in hydrogel containing 43.5% gelatin crosslinked with glutaraldehyde. Compared to the free enzyme which has a broad pH-activity profile (6.5-8.0), the activity of the immobilized enzyme is strongly dependent on pH and has a pH-optimum of pH 7.5. The optimum temperature of enzyme activity increases from 37 to 50 degrees C. Compared to the free enzyme which loses all its activity in 72 h at 50 degrees C, the immobilized enzyme retains its activity in full. The immobilized enzyme has been used efficiently in a spinning basket bioreactor for the degumming of rice bran oil with 6 recycles without loss of enzyme activity. The phosphorus content of the oil decreases from 400 ppm to 50-70 ppm in each cycle. After charcoal treatment and dewaxing, a second enzymatic treatment brings down the phosphorus content to < 5 ppm.
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