Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 136, Issue 23, Pages 8430-8437Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja503356q
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Funding
- National Research Foundation of The Ministry of Science, ICT & Future Planning in Korea [2009-0081566, NRF-2012R1A6A3A03037981]
- Cooperative Research Program for Agriculture Science & Technology Development [PJ008959]
- U.S. National Institutes of Health [CA 68687]
- Robert A. Welch Foundation [F-1018]
- National Research Foundation of Korea [2009-0081566, 2012R1A6A3A03037981] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Thioredoxin (Trx) is a redox-active protein that plays a key role in mitigating the effects of oxidative stress. The secretion of Trx on the plasma membrane has been suggested as a distinctive feature of inflammation. However, selective monitoring of membrane-associated Trx activity has proved challenging because of the ubiquity of Trx action in cells. Here, we report a Trx-specific probe that allows visualization of Trx activity associated with the membranes via fluorescence microscopy. The ability of this probe to act as a possible screening tool for agents that modulate Trx secretion was demonstrated in HeLa cells under oxidative stress conditions and in a cellular hepatosteatosis model. Control experiments serve to confirm that the response seen for the present probe is due to Trx and that it is selective over various potentially competing metabolites, including thiol-containing small molecules and test proteins.
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