Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 40, Pages 14956-14959Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja408524j
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Funding
- 973 Program [2013CB933800]
- National Natural Science Foundation of China [21227005, 21035003, 21205073]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20113704130001]
- Program for Changjiang Scholars and Innovative Research Team in University
- Science and Technology Development Programs of Shandong Province of China [2010G0020243]
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Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O-2(center dot-)) is the precursor of other ROS, exploring O-2(center dot-). fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O-2(center dot-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O-2(center dot-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O-2(center dot-). concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O-2(center dot-). in live cells and in vivo.
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