Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 47, Pages 17683-17686Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja408104w
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Funding
- Deutsche Forschungsgemeinschaft [FOR 1279, DFG RE3101/1]
- National Institutes of Health Nanomedicine Development Center for the Optical Control of Biological Function [PN2EY018241]
- NRSA [1F32EY022840]
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The use of azobenzene photoswitches has become a dependable method for rapid and exact modulation of biological processes and material science systems. The requirement of ultraviolet light for azobenzene isomerization is not ideal for biological systems due to poor tissue penetration and potentially damaging effects. While modified azobenzene cores with a red-shifted cis-to-trans isomerization have been previously described, they have not yet been incorporated into a powerful method to control protein function: the photoswitchable tethered ligand (PTL) approach. We report the synthesis and characterization of a red-shifted PTL, L-MAG0(460), for the light-gated ionotropic glutamate receptor LiGluR In cultured mammalian cells, the LiGluR +L-MAG0(460) system is activated rapidly by illumination with 400-520 nm light to generate a large ionic current. The current rapidly turns off in the dark as the PTL relaxes thermally back to the trans configuration. The visible light excitation and single-wavelength behavior considerably simplify use and should improve utilization in tissue.
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