Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 14, Pages 5290-5293Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja401075s
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Funding
- NSF CAREER
- U.S. Air Force YIP
- Norman Hackman Advanced Research Program
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1150478] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1461705] Funding Source: National Science Foundation
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Protein hydrogels have important applications in tissue engineering, drug delivery, and biofabrication. We present the development of a novel self-assembling protein hydrogel triggered by mixing two soluble protein block copolymers, each containing one half of a split intein. Mixing these building blocks initiates an intein trans-splicing reaction that yields a hydrogel that is highly stable over a wide range of pH (6-10) and temperature (4-50 degrees C), instantaneously recovers its mechanical properties after shear-induced breakdown, and is compatible with both aqueous and organic solvents. Incorporating a docking station peptide into the hydrogel building blocks enables simple and stable immobilization of docking protein-fused bioactive proteins in the hydrogel. This intein-triggered protein hydrogel technology opens new avenues for both in vitro metabolic pathway construction and functional/biocompatible tissue engineering scaffolds and provides a convenient platform for immobilizing enzymes in industrial biocatalysis.
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