Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 46, Pages 17294-17297Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja409708e
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Funding
- National Institutes of Health [R01GM093279]
- Office of Naval Research [N000141210620]
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We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric a-helical protein cosegregates with the cholesterol-poor domains in phase separating ternary mixtures.
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