Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 22, Pages 8222-8226Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja311107p
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Funding
- German Research Foundation [DEG: CRC 969, TP A5]
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Protein mutants are studied in a variety of contexts in the life sciences. However, individual mutations need to be generated in order to transcribe and translate the respective protein variants. Here, we introduce a novel strategy for controlling the incorporation of different amino acids in response to an amber stop codon by utilizing switchable designer transfer RNAs in Escherichia coli.
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