Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 10, Pages 3812-3814Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja400270a
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Funding
- Spanish grants [SAF2010-20822-C02, CTQ2012-31341, CSD2007-00006]
- Consolider Ingenio
- Xunta de Galicia [GRC2010/12, INCITE09 209084PR, PGIDIT08CSA-047209PR]
- Spanish Ministerio de Educacion
- Xunta de Galicia
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We report the rational design of a 20-mer basic peptide, derived from the transcriptional antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA[Tb3+] macrocyclic complex and a sensitizing tryptophan antenna. Folding of this peptide into an a helical conformation, which occurs upon binding to its target boxB RNA hairpin, results in a large increase in luminescence emission. Therefore, the peptide construct works as a highly sensitive and selective probe for this RNA hairpin.
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