Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 43, Pages 16018-16021Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja408093p
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Funding
- DGICYT-Spain [BQU-CTQ2012-30930]
- Generalitat de Catalunya [2009SGR 1024]
- Grupo Ferrer (Barcelona, Spain)
- Medical Research Council
- FP7Marie Curie Career Integration Grant
- Wellcome Trust [100104/Z/12/Z]
- Wellcome Trust
- MRC [G0901697] Funding Source: UKRI
- Medical Research Council [G0901697] Funding Source: researchfish
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Multicomponent reactions are excellent tools to generate complex structures with broad chemical diversity and fluorescent properties. Herein we describe the adaptation of the fluorescent BODIPY scaffold to multicomponent reaction chemistry with the synthesis of BODIPY adducts with high fluorescence quantum yields and good cell permeability. From this library we identified one BODIPY derivative (PhagoGreen) as a low-pH sensing fluorescent probe that enabled imaging of phagosomal acidification in activated macrophages. The fluorescence emission of PhagoGreen was proportional to the degree of activation of macrophages and could be specifically blocked by bafilomycin A, an inhibitor of phagosomal acidification. PhagoGreen does not impair the normal functions of macrophages and can be used to image phagocytic macrophages in vivo.
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