Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 20, Pages 7761-7767Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja4028082
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Funding
- National Science Foundation [CHE-1306928]
- NAID of the National Institutes of Health [1 R43 AI074163]
- NCI of the National Institutes of Health [R01 CA133592-01]
- Direct For Mathematical & Physical Scien [1306928] Funding Source: National Science Foundation
- Division Of Chemistry [1306928] Funding Source: National Science Foundation
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The sensitive detection of cancer biomarkers in urine could revolutionize cancer diagnosis and treatment. Such detectors must be inexpensive, easy to interpret, and sensitive. This report describes a bioaffinity matrix of viruses integrated into PEDOT films for electrochemical sensing of prostate-specific membrane antigen (PSMA), a prostate cancer biomarker. High sensitivity to PSMA resulted from synergistic action by two different ligands to PSMA on the same phage particle. One ligand was genetically encoded, and the secondary recognition ligand was chemically synthesized to wrap around the phage. The dual ligands result in a bidentate binder with high-copy, dense ligand display for enhanced PSMA detection through a chelate-based avidity effect. Biosensing with virus-PEDOT films provides a 100 pM limit of detection for PSMA in synthetic urine without requiring enzymatic or other amplification.
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